Table 4.
Differentially expressed transcripts between the RsSGL1 and RsSGL2 cDNA libraries.
| Number of Contig | Best match of Over expressed Cluster to NR protein database Probable secreted class of protein | E value | No of ESTs RsSGL1 | No of ESTs RsSGL2 | Expected | p value χ2 test |
|---|---|---|---|---|---|---|
| Protease inhibitor domain-containing | ||||||
| Contig-20 | gi67968373 chimadanin Thrombin inhibitor [Haemaphysalis longicornis] |
3e-006 | 16 | 1 | 12.85 | 0.00094 |
| Lipocalins | ||||||
| Contig-4 | gi82791912 putative serotonin and histamine binding protein [Rhipicephalus haemaphysaloides haemaphysaloides] | 8e-068 | 8 | 17 | 18.90 | 0.00067 |
| Contig-6 | gi82791912 putative serotonin and histamine binding protein [Rhipicephalus haemaphysaloides haemaphysaloides] | 3e-067 | 1 | 12 | 9.83 | 0.00087 |
| Contig-32 | gi68131541 hypothetical protein [Haemaphysalis longicornis] |
5e-006 | 9 | 0 | 6.80 | 0.00908 |
| Glycine rich proteins/other cement related sequences | ||||||
| Contig-23 | gi20069012salivary gland-associated protein 64P [Rhipicephalus appendiculatus] |
2e-022 | 1 | 13 | 10.58 | 0.00051 |
| Immunomodullatory-antiinflammatory proteins | ||||||
| Contig-60 | gi215275255EVA3_RHISA RecName: Full = Evasin-3 | 1e-033 | 6 | 0 | 4.53 | 0.03316 |
| Other putative salivary peptides | ||||||
| Contig-17 | gi215497897 secreted protein, putative [Ixodes scapularis] |
8e-005 | 24 | 5 | 21.93 | 0.00030 |
| Contig-40 | gi76786687 putative secreted protein [R. microplus] |
0.002 | 8 | 0 | 6.05 | 0.01391 |
RsSGL1 - salivary gland cDNA library made from female ticks fed for 3-5 days (early to mid library).
RsSGL2 - salivary gland cDNA library made from female ticks fed for 5 days (mid library).