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. 1993 Apr 25;21(8):1819–1826. doi: 10.1093/nar/21.8.1819

Design and synthesis of polyacrylamide-based oligonucleotide supports for use in nucleic acid diagnostics.

E Fahy 1, G R Davis 1, L J DiMichele 1, S S Ghosh 1
PMCID: PMC309420  PMID: 7684127

Abstract

Polyacrylamide supports, in a range of pore sizes, were investigated as nucleic acid affinity matrices for the detection of target DNA or RNA sequences using a sandwich hybridization format. Bromoacetyl and thiol oligonucleotide derivatives were covalently linked to sulfhydryl- and bromoacetyl-polyacrylamide supports with greater than 95% end-attachment efficiencies. These polyacrylamide-oligonucleotide supports were further derivatized with anionic residues to provide multi-functional supports which show low non-specific binding for non-complementary nucleic acids. While all the polyacrylamide-oligonucleotide supports capture complementary oligonucleotides with high affinity, the pore size was found to be a critical parameter in sandwich hybridization reactions. The superior hybridization characteristics of the Trisacryl support was ascribed to a combination of its macroporous nature, hydrophilicity and the terminal attachment of its capture oligonucleotides.

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Selected References

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