Table 1.
Fyn and Src kinase activities in the forebrain of fyn transgenic mice
| Mouse line
|
Fyn kinase activity (fmoles/min/μg− ± s.e.m.)
|
Src kinase activity (fmoles/min/μg ± s.e.m.)
|
||
|---|---|---|---|---|
|
fyn+/+ background
|
fyn−/− background
|
sp. act.a
|
||
| Nontransgenic | ||||
| wild-type | 1.91 ± 0.29 (7) | — | 1.86 ± 0.29 | 3.54 ± 0.18 (6) |
| fyn− | — | 0.01 ± 0.03 (4) | — | 6.33 ± 0.27 (4) |
| Native fyn transgenic | ||||
| N 8 | 16.47 ± 1.40 (7) | 13.44 ± 1.37 (6) | 2.27 ± 0.21 | 3.21 ± 0.29 (6) |
| N 39 | 5.63 ± 0.96 (7) | 3.76 ± 0.54 (6) | 1.81 ± 0.24 | 3.60 ± 0.60 (3) |
| N 85 | 14.20 ± 0.95 (7) | 9.41 ± 1.29 (4) | 2.52 ± 0.30 | 3.52 ± 0.28 (6) |
| N 92 | 13.21 ± 0.91 (7) | 9.75 ± 0.74 (6) | 2.03 ± 0.14 | 3.43 ± 0.48 (6) |
| Mutant fyn transgenic | ||||
| M 27 | 4.08 ± 0.50 (7) | 1.72 ± 0.22 (6) | 1.56 ± 0.18 | 3.79 ± 0.06 (3) |
| M 58 | 3.77 ± 0.25 (7) | 1.00 ± 0.14 (6) | 2.44 ± 0.35 | 3.78 ± 0.31 (3) |
| M 78 | 5.50 ± 0.56 (7) | 3.30 ± 0.32 (6) | 1.71 ± 0.12 | 3.61 ± 0.38 (3) |
Fyn kinase activity of RIPA extract of forebrain was measured in wild-type mice, fyn knockout mice (fyn−) and several lines of native and mutant fyn transgenic mice generated on either wild-type (fyn+/+) or fyn knockout (fyn−/−) genetic background. Src kinase activity was also measured in each group of mice, wild-type mice, fyn knockout mice (fyn−), and several lines of native and mutant fyn transgenic mice generated on wild-type (fyn+/+) genetic background. Value represents the mean ± s.e.m.. Total number of animals examined is represented in a parenthesis.
The specific activity was determined by dividing the kinase activity, which was measured in wild-type mice and in fyn transgenic mice on fyn knockout genetic background, from the relative amount of Fyn protein estimated by immunoblotting (Fig. 2).