Fig. 6. PX1 knock-down (KD) in TM5 cells by lentivirus-mediated RNA interference.
The successful KD of PX1 mRNA and protein in TM5 cells was verified by real-time PCR (A) and semi-quantitative immunoblotting (B), respectively. The stable PX1 KD in the TM5 cell line lasted over 2 months after transduction (A). N refers to numbers of independent experiments performed. *P< 0.01 compared with mock controls (MC) by Student’s t-test.