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. Author manuscript; available in PMC: 2011 Jul 12.
Published in final edited form as: Nat Neurosci. 2009 Feb 22;12(3):311–317. doi: 10.1038/nn.2275

Figure 4. Conditioned medium from MeCP2-null astrocytes cannot support normal neuronal growth.

Figure 4

Co-immunostaining of hippocampal neurons with MAP2 as dendritic marker (red) and MeCP2 (green). (a) Hippocampal neurons from WT mice cultured for 6 days in astrocytic conditioned media (ACM) from wild-type (WT), MeCP2-null astrocytes (Mut), or with mixed ACM from WT and mutant astrocytes. (b) Bar graphs represent the fraction of neurons with at least two short (>50 mm) dendrites when cultured in the different ACM. Error bars represent SD based on three independent experiments. (c) WT hippocampal neurons cultured for 7 days with conditioned medium generated from MeCP2-null astrocytes of the Bird mouse model show similar abnormal morphology. (d) Bar graphs as in b. (e) Hippocampal neurons from RTT mice (Mut) cultured for 6 days are supported by conditioned medium from WT astrocytes (WT ACM). (f) Bar graphs as in b. Note that the gain of image in Mut hippocampal neurons is increased for MeCP2 because MeCP2-null neurons from the Jaenisch mouse model express low levels of the C-terminus, which is recognized by the anti-MeCP2 antibody used. Calibration bars, 40 μm.