| Ligation mix from step 2 | 1ul |
| 5x phusion buffer | 10ul |
| PE1.0 | 1ul |
| PE2.0 | 1ul |
| 25mM dNTP | 0.5ul |
| Phusion | 0.5ul |
| H2O | 36ul |
| Total | 50ul |
| 98°C 30 sec | |
| 98°C 10 sec | |
| 65°C 30 sec | |
| 72°C 30 sec | |
| 72°C 5 min | |
| 4°C ∞ |
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