Figure 9.
Primary lung epithelial cell growth is impaired by imatinib. Cells expressing epithelial cell adhesion molecule (EpCAM+ cells) were isolated from the lung by flow cytometric sorting and grown in culture with (A) control or (B) imatinib at 9.1 μM. The average radius of all colonies for each condition was determined. (C) The mean radius of colonies is reduced by imatinib (2.7 and 9.1 μM) when compared with saline treatment (*P < 0.01). (D) Number of colonies was characterized by radius, and the number of large colonies was greater with saline treatment when compared with imatinib (9.1 μM). EpCAM+ cells were isolated from the lung by flow cytometric sorting and grown in culture with (E) control or (F) stem cell factor at 50 ng/ml. (G) Number of colonies was characterized by radius, and the number of large colonies was greater with stem cell factor treatment (6.25–100 ng/ml) when compared with control.