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. Author manuscript; available in PMC: 2011 Jul 21.
Published in final edited form as: Nature. 2009 May 7;459(7243):118–121. doi: 10.1038/nature08034

Figure 2. Translation elongation defect in temperature-sensitive tif51a-D63V mutant.

Figure 2

(a) Isogenic WT (J697) and tif51a-D63V mutant (D63V; J698) strains were serially diluted, spotted on SC medium, and incubated 3 days at 25, 37, or 38 °C. (b) WT (left panel) and tif51a-D63V (right) mutant strains were shifted to 36 °C for 2 h, and then labeled with [35S]Met. Fitting lines of total (filled symbols) and completed (open symbols) protein synthesis were obtained by linear regression and used to determine the half-average (t1/2) and average (T) transit time. Data are the average of three independent experiments. (c) In vitro translation activity of heat-treated extracts from WT and tif51a-D63V strains following addition of eIF5A or eIF5AK51R. Results are representative of six independent experiments.