Fig. 2.
Positional cloning of the LUT1 locus. (A) Fine-mapping of the interval containing LUT1. Recombinants are indicated for specific simple sequence length polymorphism markers across the interval, and the position of chloroplast-targeted proteins are indicated by dashed arrows. BACs, bacterial artificial chromosomes. (B) Overview of the intron–exon organization of LUT1 and the locations of the lut1-1 and lut1-3 mutations. (C) Deduced amino acid sequence of LUT1. The cleavage site of the putative chloroplast-targeting sequence is indicated by an arrow, and the single predicted transmembrane domain is shaded in black. The conserved cytochrome P450 molecular oxygen-binding pocket and the cysteine motif are indicated by single and double underlines, respectively, and the conserved Thr is indicated by an asterisk.