Figure 3.
PI3 kinase and PKC-ζ are required for insulin-stimulated polyadenylation. (A) Oocytes were incubated with wortmannin for 2 h and then in some cases, injected with CPE-containing RNA, which was followed by stimulation with progesterone or insulin. MPF activity, mos protein levels, CPEB phosphorylation, and cytoplasmic polyadenylation were then assessed as described in Fig. 1. (B) Oocytes were incubated with rapamycin for 2 h, injected with CPE-containing RNA, and the same parameters as in panel A were assayed. (C) Oocytes were injected with PKC-ζ inhibitor peptide, PKC-α inhibitor peptide, or mock-injected, and induced to mature with insulin or progesterone. The same parameters as in A were assayed.