Table 2.
Polarity relief at phage tR1 terminator in the nusA(R258C) mutant and its suppression by H-NSΔ64 or YdgT expression
| Straina | Mean ± SE |
||
|---|---|---|---|
| β-Galactosidase sp act |
% B/A | ||
| –tR1 (A) | +tR1 (B) | ||
| Wild type | 9,400 ± 2,151 | 425 ± 79 | 5 ± 1 |
| nusA | 7,045 ± 1,451 | 1,327 ± 207 | 19 ± 4 |
| nusA/vector | 7,853 ± 1,335 | 1,157 ± 22 | 15 ± 3 |
| nusA/H-NSΔ64 | 9,995 ± 1,985 | 532 ± 21 | 5 ± 1 |
| nusA/YdgT | 4,000 ± 7 | 246 ± 1 | 6 ± 0 |
a
The strains (grown in LB medium) included those without (−tR1) or with (+tR1) the phage tR1 terminator upstream of the lacZ reporter gene and were, respectively: wild type, RS445 and GJ5147, and nusA, GJ10573 and GJ10572. Derivatives with vector, H-NSΔ64, and YdgT carried plasmids pLG339, pLG-H-NSΔ64, and pHYD2546, respectively.