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. 2011 Sep 1;138(17):3625–3637. doi: 10.1242/dev.064162

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Fig. 6. — During muscle regeneration, ablation of Tcf4⁺ fibroblasts leads to premature satellite cell differentiation and smaller regenerated myofibers. (A-L) At 3 dpi, Tcf4⁺ cells are reduced (J-L), with no change in Pax7⁺ cells (A-C), but with an increase in MyoD⁺ progenitors/myoblasts (D-F) and MyHCemb⁺ regenerating myofibers (G-I) in Tcf4^CreERT2/+;R26R^DTA/+ mice. (M-X) At 5 dpi, 42% of Tcf4⁺ cells were calculated to be ablated (V-X), resulting in fewer Pax7⁺ cells (M-O), MyoD⁺ progenitors/myoblasts (P-R) and MyHCemb⁺ regenerating myofibers (S-U) in Tcf4^CreERT2/+;R26R^DTA/+ mice. (Y-KK) At 28 dpi, despite Tcf4⁺ fibroblast ablation (HH-JJ), Pax7⁺ cells recover (Y-AA), muscle largely regenerates (BB-GG), but diameter of myofibers is reduced (BB,CC,EE,FF,KK) in Tcf4^CreERT2/+;R26R^DTA/+ versus Tcf4^+/+;R26R^DTA/+ mice. (LL-PP) By 56 dpi, regenerated muscle is recovered in Tcf4^CreERT2/+;R26R^DTA/+ mice. Scale bars: in OO,100 μm for all photomicrographs. For all graphs, mean ± s.e.m. are plotted.