| For 1 cycle: | 95°C | 30 sec |
| For 5 cycles: | 95°C | 5 sec |
| 72°C | 2 min | |
| For 5 cycles: | 95°C | 5 sec |
| 70°C | 10 sec | |
| 72°C | 2 min | |
| For 30 cycles: | 95°C | 5 sec |
| 68°C | 10 sec | |
| 72°C | 2 min |
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