FIGURE 2.
CCL3 expression is increased in TTP−/− mouse bone marrow-derived macrophages (BMDM). A, CCL3 mRNA levels after LPS (10 ng/ml) stimulation are higher in TTP−/− compared to TTP+/+ BMDM. The time course of TTP protein induction by LPS treatment is shown by western blotting. B, CCL3 protein in the medium was quantified by ELISA. C, CCL3 and TNF mRNA half-lives are increased in TTP−/− versus TTP+/+ BMDM. After stimulating with LPS for 90 min, actinomycin D (Act. D) was added to the cells. At the indicated times (Time after Act. D), mRNA was isolated from the cells and quantified by RT-PCR. Dashed line indicates 50% level. Data presented as mean ± SEM, n = 3 to 6.