Figure 2.
LIN-3 is expressed in epithelial cells of adults. To examine LIN-3 expression, we obtained transgenic syIs107 nematodes that express GFP protein with a nuclear localization signal from a genomic lin-3 promoter. (A) L2 animal showing expression (arrows) from lin-3 promoter in the pharynx. Bar, 100 μm. (B) L3 animal showing expression from the lin-3 promoter in the anchor cell along the ventral mid-body (the dorsal and ventral cuticle is outlined in white). L4 animals later show vulF cell expression in this same region of the body. Bar, 50 μm. While not detected in larvae, there is epithelial expression from the lin-3 promoter in adults starting at L4+24 h in (C) the intestine and (D) the hypodermis. Bar, 100 μm.