Table 2.
tam-1 effects on reporter transgene activity
|
|
Construct
|
Relative intensities of reporters
|
||
|---|---|---|---|---|
| expression pattern
|
wild-type
|
tam-1
|
||
| A | myo-3::gfp | body-wall muscle vulval muscle | ++++ +++ | + + |
| unc-54::gfp | body-wall muscle | +++ | + | |
| hlh-1::gfp | body-wall muscle | ++ | − | |
| myo-2::gfp | pharyngeal muscle | ++++ | ++ | |
| F22B7.9::gfp | gut | ++ | − | |
| egl-15::gfp | vulval muscle | +++ | − | |
| gut | + | − | ||
| neurons near head | + | − | ||
| lin-3::lacZ | anchor cell | +++ | + | |
| gpa-1::lacZ | head neurons | +++ | + | |
| plasmid neurons | +++ | + | ||
| lin-11::lacZ | 2° vulval lineages | ++ | − | |
| VC motor neurons | +++ | − | ||
| ventral uterine π cells and their progeny | ++ | − | ||
| mab-5::gfp | posterior region neurons near tail | + + | −− | |
| B | let-858::gfp | all somatic nuclei | +++ | +++ |
| rps-5::gfp | all somatic nuclei | +++ | +++ | |
| glr-1::gfp | subset of neurons | +++ | +++ | |
| unc-119::gfp | many neurons | +++ | +++ | |
Expression of tandemly repeated transgenes in wild-type and tam-1 mutants. GFP transgenes were examined in tam-1(cc567), and lacZ transgenes were examined in tam-1(sy272). Number of plus signs (+) indicate relative intensity of GFP fluorescence or lacZ signal. (−) No observable signal. In cases where no expression was observed in tam-1 mutants, an outcross was performed to confirm the reappearance of reporter expression. (A) tam-1 effects were seen on a number of different reporter constructs. (B) Other transgenes showed little or no effect in tam-1 mutants. Additional myo-3::gfp and rps-5::gfp transgenes (independently derived) were tested in wild-type and tam-1(cc567) backgrounds and showed similar results (data not shown).