Table 1.
A hog1 mutation suppresses the mating defects of ste4 and ste5 strains in the presence of 1 m sorbitol
|
a strainsa
|
YEPD mating (%)b
|
YEPD + 1 m sorbitol mating (%)b
|
Effect of 1 m sorbitolc
|
|---|---|---|---|
| Wild type | 58 ± 5 | 74 ± 12 | 1.3 |
| ste4 | 1.4 ± 0.4 × 10−5 | 6.8 ± 2 × 10−5 | 4.9 |
| ste5 | 4.7 ± 2 × 10−6 | 6.6 ± 2 × 10−6 | 1.4 |
| ste20 | 2.9 ± 0.6 | 0.79 ± 0.3 | 0.27 |
| ste11 | <3.3 ± 2 × 10−6 | 5.3 ± 2 × 10−6 | — |
| hog1 | 68 ± 7 | 63 ± 9 | 0.93 |
| hog1 ste4 | 2.7 ± 1 × 10−5 | 1.8 ± 0.3 × 10−2 | 670 |
| hog1 ste5 | 1.6 ± 0.6 × 10−5 | 0.26 ± 0.08 | 16,000 |
| hog1 ste20 | 6.6 ± 2 | 9.4 ± 3 × 10−2 | 0.014 |
| hog1 ste11 | <3.8 ± 1 × 10−6 | 1.2 ± 0.7 × 10−5 | — |
| hog1 sho1 | 72 ± 8 | 61 ± 9 | 0.85 |
| hog1 sho1 ste4 | 3.5 ± 2 × 10−5 | 3.1 ± 0.8 × 10−5 | 0.89 |
| hog1 sho1 ste5 | 2.0 ± 2 × 10−5 | 6.1 ± 4 × 10−5 | 3.1 |
a
Strains tested were SO329 (wild type), SO341 (ste4), IH2731 (ste5), IH2735 (ste20), SO336 (ste11), SO330 (hog1), SO331 (hog1 ste4), SO332 (hog1 ste5), SO391 (hog1 ste20), SO333 (hog1 ste11), SO356 (hog1 sho1), SO344 (hog1 sho1 ste4), and SO398 (hog1 sho1 ste5).
b
Mating efficiency is the number of diploids formed divided by the number of total cells at the end of the experiment. Strains were mated to wild-type α strain IH1793.
c
The effect of 1 m sorbitol was calculated by dividing the mating efficiency on YEPD + 1 m sorbitol by the mating efficiency on YEPD.