Fig. 2.
ASC−/− animals are protected against HFD-induced insulin resistance, steatosis, and adipocyte hypertrophy. (A) Insulin tolerance test (ITT) and glucose tolerance tests (GTT) of HFD-fed wild-type and ASC−/− animals. (B) Liver triglyceride (Tg) levels in HFD-fed wild-type and ASC−/− mice. (C) Liver histology as determined by H&E staining. (Scale bars, 100 μm.) (D) Epididymal adipose tissue weight of LFD- and HFD-fed wild-type or ASC−/− mice. (E) Adipose tissue morphology after H&E staining of HFD-fed wild-type and ASC−/− mice. (Scale bars, 100 μm.) (F and G) Quantification of adipocyte size using software analysis. (H) Localization of macrophages in WAT of HFD-fed wild-type and ASC−/− at week 16. Arrow indicates macrophage specific immunoreaction. (Scale bars, 100 μm.)