Table 1.
Electrophysiological properties of layer 2/3 pyramidal cells expressing GECIs.
| Electrophysiological property | YC2.60 (n = 22) | YC3.60 (n = 16) | YC-Nano15 (n = 14) | GCaMP3 (n = 7) | no expression (n = 11) | p-value | |
|---|---|---|---|---|---|---|---|
| Vma | (mV) | −83 ± 5.4 | −82 ± 7.4 | −81 ± 6.5 | −89 ± 4.1 | −82 ± 8.7 | 0.14 |
| Rmb | (MΩ) | 203 ± 78 | 191 ± 53 | 209 ± 59 | 208 ± 81 | 139 ± 42 | 0.061 |
| AP amplitudec | (mV) | 105 ± 10 | 98 ± 12 | 100 ± 6.8 | 108 ± 8.2 | 107 ± 19 | 0.18 |
| AP thresholdd | (mV) | −47 ± 3.3 | −45 ± 5.4 | −46 ± 5.4 | −50 ± 3.1 | −47 ± 7.4 | 0.37 |
| AP half widthe | (ms) | 2.3 ± 0.8 | 2.7 ± 1.7 | 2.5 ± 0.6 | 1.6 ± 0.5 | 1.7 ± 0.5 | 0.055 |
All values are corrected for liquid junction potential (12 mV).
aResting membrane potential.
bInput resistance.
cAmplitude of action potential measured from the resting membrane potential.
dThreshold voltage for action potential generation defined as the point where the first temporal derivative of the voltage first exceeds 50 mV/ms.
eFull width of action potential measured at half the amplitude.