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. 2011 Nov 1;22(21):4192–4204. doi: 10.1091/mbc.E11-05-0467

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© 2011 Hickman et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Increasing intracellular levels of SAM suppresses the growth defect of met4Δ. (A) Adding SAM to YPD rescues growth of a met4Δ haploid. A met4Δ/MET4 heterozygote (DBY12043) was sporulated, and the resulting tetrads were dissected on YPD plates with 0.2 mM SAM. Many SAM concentrations (0.05–0.5 mM) were tested, with 0.2 mM supporting the optimal met4Δ growth. The met4Δ cells remained, as expected, methionine auxotrophs. (B) Overexpression of the SAM2 gene rescues growth of a met4Δ haploid. A met4Δ/MET4 GAL1_pr-SAM2/SAM2 heterozygote (DBY12233) was sporulated, and the resulting tetrads were dissected on YPD or YPGal plates.