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. Author manuscript; available in PMC: 2012 Nov 18.
Published in final edited form as: Mol Cell. 2011 Nov 18;44(4):597–608. doi: 10.1016/j.molcel.2011.09.016

Figure 7. ATM phosphorylates Bub1 on Ser314 to activate the spindle checkpoint.

Figure 7

Isogenic HeLa cell lines with proficient (control shRNA) or deficient (ATM shRNA) ATM were treated with mock or nocodazole and subjected to immunoblotting with the indicated antibodies. (C) HeLa cells transfected with vector, WT or S1403A ATM were treated with nocodazole and immunoblotted with the indicated antibodies. (D) HeLa cells were transfected with vector, WT or S314A Bub1. 36 h after transfection, cells were treated with nocodazole and subjected to flow cytometry analysis. The averages of the mitotic index of at least three independent samples are shown. Error bars are standard deviations.