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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Cell. 2011 Apr 1;145(1):145–158. doi: 10.1016/j.cell.2011.03.012

Figure 6. APC loss is required for the maintenance of T lymphoblastic leukemia/lymphoma.

Figure 6

(A) Kaplan Meier analysis of R26-rtTA/TG-shRNA double transgenic mice treated continually with DOX from 4–6 weeks of age.

(B) Fluorescent image of TG-APC.9365/R26-rtTA animals with GFP positive tumor in the thymus. All analyzed animals that became moribund developed T lymphoblastic leukemia/lymphoma apparent in the thymus, lymph nodes (not shown) and liver. Scale bars: 50μm

(C) Representative flow cytometry plots of cells isolated from primary lymphomas and stained for surface markers: Thy1, CD4 and CD8

(D) GFP positive (left) and CD4/CD8 double positive cells in the peripheral blood of SCID mice transplanted with TG-APC.3374 lymphoma following the DOX-switch. Error bars = SEM.

(E) H&E stained sections of the liver (upper) and bone marrow (femur – lower) in mice on DOX or removed from DOX for 2 weeks (off DOX). Scale bars: 50μm (liver) and 20μm (femur).

(F) Kaplan Meier analysis of SCID mice transplanted with TG-APC.3374 primary lymphoma. Graph is plotted from the time of DOX-switch (5 weeks after transplant).

See also Figure S6