Figure 6.
InternalizedIL-7Rα localizes primarily within Vps36 vesicles. Freshly isolated T cells were fixed, permeabilized and stained with indicated antibodies for fluorescence microscopy. (A) Vps34 colocalizes with HRS in MVB. (B) IL-7Rα is enriched near the Golgi apparatus. (C) Vps36 colocalizes with IL-7Rα intracellularly, and to a lesser extent, with TGN46 in WT T cells. (D) Quantification of IL-7Rα and Vps36 colocalization (n=20). (E) Colocalization between IL- 7Rα and Vps35 (n=20). Data are representative of two experiments. (C, D, and E) Vps34−/− = Vps34f/fLck-cre