Figure 7.
Defective IL-7Rα recovery in Vps34f/fLck-cre T cells that is dependent on vesicle trafficking. (A) IL-7Rα expression on Vps34f/fLck-cre T cells post-pronase treatment. Vps34f/fLck-cre CD4+ T cells were treated twice with 0.08% pronase for 15 minutes, washed, and allowed to recover for indicated times, in the presence or absence (B) of cycloheximide (CHX) or cytochalasin B (cyto B). Gray histograms represent IL-7Rα surface levels at time 0, immediately after pronase treatment and indicative of pronase efficacy. (C) Values from (A) and (B) are calculated, normalized to starting IL-7Rα surface expression. n=4; ND=Not determined. Data are representative of two independent experiments. (A, B, and C) Vps34−/− = Vps34f/fLck-cre