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. 2012 Jan 1;23(1):188–199. doi: 10.1091/mbc.E11-07-0638

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© 2012 Pedram et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — ARs are substrates for DHHC-7 and -21. (A) Transfection of individual DHHC-encoding plasmids into C4-2 prostate cancer cells was followed by [³H]palmitic acid labeling. Immunoblots for AR total protein and HA-tagged DHHC PAT proteins are shown. A representative experiment repeated two more times is shown. (B) Distribution of AR in C4-2 cells. Left, cells were transfected with the siRNAs and Western blots of cell fractions were performed. Knockdown of DHHC-7 or -21 decreased membrane, but not nuclear, localization of the steroid receptor. Bar graphs are combined mean ± SEM membrane AR densities from three experiments. *, p < 0.05 for control siRNA vs. DHHC siRNA. (C) DHHC-7 or -21 siRNA significantly reduced [³H]palmitate incorporation into endogenous AR. Total AR protein is shown. Bar graphs are data from three experiments. *, p < 0.05 for control siRNA vs. DHHC siRNA.