Fig. 4.
Double-knockout Px1−/−Px2−/− mice were protected in a stroke model. (A, B, D, and E) Compared with Px1+/+Px2+/+ littermates, neurological deficits did not differ in Px1−/− and Px2−/− mice after MCAO as evaluated by the corner test (A and B). When measuring latency to move, Px1−/− mice showed a trend (D) and Px2−/− a significantly shorter latency than wild-type littermates (E), F(1/58) = 9.7, P < 0.01. **P < 0.01 (repeated-measures ANOVA). The dashed line indicates the expected behavior without a side preference in 12 trials of the corner test. Mice were investigated before and 24 h after MCAO. Values are mean ± SEM (n = 6–14). (G and H) Px1−/− and Px2−/− mice showed no statistically significant differences in infarct volume compared with Px1+/+Px2+/+ littermates. Values are mean ± SEM (n = 15–18). (C and F) Double-knockout Px1−/−Px2−/− mice had a smaller neurological deficit 24 h after MCAO than Px1+/+Px2+/+. In the corner test (C), the pathological preference of Px1+/+Px2+/+ littermates to turn to the right side was significantly reduced in Px1−/−Px2−/− mice 24 h after MCAO. F(1/12) = 6.7, P < 0.05. *P < 0.05 (repeated-measures ANOVA, n = 5–9). In the latency to move (F), Px1−/−Px2−/− mice needed significantly less time to move compared with their Px1+/+Px2+/+ littermates. F(1/12) = 5.9, P < 0.01. **P < 0.01 (repeated-measures ANOVA, n = 5–9). (I) Double deletion of both Px1 and Px2 (Px1−/−Px2−/−) significantly reduced the infarct volume compared with the Px1+/+Px2+/+ littermates. **P < 0.01 (t test, n = 6–10).