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. 1983 Jul 25;11(14):4939–4946. doi: 10.1093/nar/11.14.4939

Strong sequence conservation of a 38 bp region near the center of the extrachromosomal rDNA palindrome in different Tetrahymena species.

J Engberg
PMCID: PMC326097  PMID: 6308565

Abstract

The restriction-endonuclease map and the nucleotide sequence of the central region in the extrachromosomal rDNA palindrome of two micronuclear and one a-micronucleate species of Tetrahymena has been determined. The sequence data show that the different species investigated have a 24 or 26 nucleotide sequence region at the very center of the rDNA molecule which is non-palindromic. Comparison of the present sequence data with the published data of another micronucleate species reveal that a segment of 38 base pairs just outside the non-palindromic center is highly conserved in all the different species, while the rest of the central region show little sequence homology. The relevance of this conserved region to the amplification process of the rDNA molecule is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cech T. R., Brehm S. L. Replication of the extrachromosomal ribosomal RNA genes of Tetrahymena thermophilia. Nucleic Acids Res. 1981 Jul 24;9(14):3531–3543. doi: 10.1093/nar/9.14.3531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Din N., Engberg J. Extrachromosomal ribosomal RNA genes in Tetrahymena: structure and evolution. J Mol Biol. 1979 Nov 5;134(3):555–574. doi: 10.1016/0022-2836(79)90367-x. [DOI] [PubMed] [Google Scholar]
  3. Engberg J., Andersson P., Leick V., Collins J. Free ribosomal DNA molecules from Tetrahymena pyriformis GL are giant palindromes. J Mol Biol. 1976 Jun 25;104(2):455–470. doi: 10.1016/0022-2836(76)90281-3. [DOI] [PubMed] [Google Scholar]
  4. Engberg J., Nasir-ud-Din, Eckert W. A., Kaffenberger W., Pearlman R. E. Detailed transcription map of the extrachromosomal ribosomal RNA genes in Tetrahymena thermophila. J Mol Biol. 1980 Sep 25;142(3):289–313. doi: 10.1016/0022-2836(80)90274-0. [DOI] [PubMed] [Google Scholar]
  5. Hartley J. L., Donelson J. E. Nucleotide sequence of the yeast plasmid. Nature. 1980 Aug 28;286(5776):860–865. doi: 10.1038/286860a0. [DOI] [PubMed] [Google Scholar]
  6. Higashinakagawa T., Saiga H., Shintani N., Narushima-Iio M., Mita T. Localization of putative transcription initiation site on the cloned rDNA fragment of Tetrahymena pyriformis. Nucleic Acids Res. 1981 Nov 25;9(22):5905–5916. doi: 10.1093/nar/9.22.5905. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kan N. C., Gall J. G. Sequence homology near the center of the extrachromosomal rDNA palindrome in Tetrahymena. J Mol Biol. 1981 Dec 25;153(4):1151–1155. doi: 10.1016/0022-2836(81)90472-1. [DOI] [PubMed] [Google Scholar]
  8. Kiss G. B., Amin A. A., Pearlman R. E. Two separate regions of the extrachromosomal ribosomal deoxyribonucleic acid of Tetrahymena thermophila enable autonomous replication of plasmids in Saccharomyces cerevisiae. Mol Cell Biol. 1981 Jun;1(6):535–543. doi: 10.1128/mcb.1.6.535. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kiss G. B., Pearlman R. E. Extrachromosomal rDNA of Tetrahymena thermophila is not a perfect palindrome. Gene. 1981 Apr;13(3):281–287. doi: 10.1016/0378-1119(81)90032-9. [DOI] [PubMed] [Google Scholar]
  10. Leick V., Engberg J., Emmersen J. Nascent subribosomal particles in Tetrahymena pyriformis. Eur J Biochem. 1970 Apr;13(2):238–246. doi: 10.1111/j.1432-1033.1970.tb00923.x. [DOI] [PubMed] [Google Scholar]
  11. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  12. Niles E. G., Jain R. K. Physical map of the ribosomal ribonucleic acid gene from Tetrahymena pyriformis. Biochemistry. 1981 Feb 17;20(4):905–909. doi: 10.1021/bi00507a039. [DOI] [PubMed] [Google Scholar]
  13. Sor F., Fukuhara H. Unequal excision of complementary strands is involved in the generation of palindromic repetitions of rho- mitochondrial DNA in yeast. Cell. 1983 Feb;32(2):391–396. doi: 10.1016/0092-8674(83)90458-0. [DOI] [PubMed] [Google Scholar]
  14. Truett M. A., Gall J. G. The replication of ribosomal DNA in the macronucleus of Tetrahymena. Chromosoma. 1977 Dec 6;64(4):295–303. doi: 10.1007/BF00294937. [DOI] [PubMed] [Google Scholar]
  15. Tschumper G., Carbon J. Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene. Gene. 1980 Jul;10(2):157–166. doi: 10.1016/0378-1119(80)90133-x. [DOI] [PubMed] [Google Scholar]
  16. Wild M. A., Gall J. G. An intervening sequence in the gene coding for 25S ribosomal RNA of Tetrahymena pigmentosa. Cell. 1979 Mar;16(3):565–573. doi: 10.1016/0092-8674(79)90030-8. [DOI] [PubMed] [Google Scholar]
  17. Yao M. C., Blackburn E., Gall J. G. Amplification of the rRNA genes in Tetrahymena. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):1293–1296. doi: 10.1101/sqb.1979.043.01.147. [DOI] [PubMed] [Google Scholar]
  18. Yao M. C., Gall J. G. A single integrated gene for ribosomal RNA in a eucaryote, Tetrahymena pyriformis. Cell. 1977 Sep;12(1):121–132. doi: 10.1016/0092-8674(77)90190-8. [DOI] [PubMed] [Google Scholar]

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