Figure 8.
MTHFS-GFP co-localizes with hTrifGART-OFP and hFGAMS-OFP under purine-depleted conditions. Fluorescent fusion proteins were expressed in HeLa cells maintained in purine-deficient RPMI as described in Section “Materials and methods.” (A) hMTHFS-GFP fusion protein, (B) hFGAMS-OFP fusion protein, (C) merge of (A) and (B). (D) hMTHFS-GFP fusion protein, (E) hTrifGART-OFP fusion protein, (F) merge of (D) and (E); nuclear stain (DRAQ5) shown in blue.