FIG. 3.
Prolongation of incubation times in intracerebrally TSE-infected mice treated with quinine or biquinoline. (A) Tg7 mice infected with 263K agent strain and intraventricularly treated with quinine; (B) Tg20 mice infected with RML agent strain and intraventricularly treated with quinine; (C) Tg20 mice infected with Fukuoka-1 agent strain and intraventricularly treated with quinine; (D) Tg7 mice infected with 263K agent strain and intraperitoneally or intraventricularly treated with biquinoline. A 4-week continuous intraventricular infusion of a chemical was initiated by using an osmotic pump at day 10 (1.5W) or day 35 (5W) post-intracerebral inoculation in Tg7 mice or at day 14 (2W) or day 49 (7W) in Tg20 mice. For intraperitoneal treatment, injection of a chemical in Tg7 mice was performed intraperitoneally once a day for 5 days per week from day 10 (1.5W) or day 35 (5W) until the death of the mouse. Each closed circle represents the incubation time of an individual mouse. Each solid line and bar represent the average and standard deviation of the incubation times of each group. The star indicates groups with results with P < 0.05 compared to the results seen with the vehicle control. Each of the experiments was performed independently using different lots of the pathogen homogenate; thus, there was some variation in the data shown in panels A and D even for the same vehicle control.