Table 1. Effect of mVL30-1 RNA on gene transcription in human cell lines.
| Gene ID | Th/Te ratio | Gene symbol | Gene description |
|---|---|---|---|
| U19147 | 10.4 | GAGE6 | G antigen 6 |
| M98528 | 5.5 | D4S234E | Neuron-specific protein |
| J02611 | 5.1 | APOD | Apolipoprotein D |
| M22637 | 4.8 | LYLI | Lymphoblastic leukemia-derived sequence 1 |
| X66435 | 4.5 | HMGCSI | 3-Hydroxy-3-methylglutaryl-coenzyme A synthase 1 (soluble) |
| AF018081 | 4.5 | COLI8A1 | Collagen, type XVIII, alpha 1 |
| X99133 | 4.4 | LCN2 | Lipocalin 2 (oncogene 24p3) |
| M14565 | 4.1 | P450scc | P-450 cholesterol side-chain cleavage enzyme |
| U33635 | 4.0 | PTK7 | PTK7 protein tyrosine kinase 7 |
| M35878 | 3.9 | IGFBP3 | Insulin-like growth factor binding protein 3 |
| L00352 | 3.8 | LDLR | Low-density lipoprotein receptor |
| Y12661 | 0.39 | VGF | VGF nerve growth factor inducible |
| X69392 | 0.38 | RPL26 | Ribosomal protein L26 |
Microarray slides containing 16,000 human ESTs were hybridized with cDNA probes prepared from the cloned human melanoma lines Th and Te. Both lines were retrovirally transfected with TF cDNA and express high levels of TF protein, but only the Th line contains mVL30-1 RNA transmitted by the retroviral vector (2). The genes corresponding to the microarray spots showing a >3.8-fold increase or 2.5-fold decrease in the Th/Te ratio were further tested by RT-PCR assays for Th and Te cells (see Fig. 1A). The genes satisfyng the criteria for both differential hybridization and differential RT-PCR are listed in Table 1.