Figure 7.
Eye phenotypes of Lam mutants. Light micrographs of apical tangential sections through adult compound eyes are shown in A, C, E, G, and I, and of sections through third instar larval eye discs immunostained with anti-Elav to label R-cell nuclei in B, D, F, H, J, and K. (A and B) Wild-type eyes and discs. The numbers in A refer to the seven R-cells in each ommatidium visible in apical sections. The R-cell nuclei are apical in B. (C and D) LamA25 homozygotes. In C, some ommatidia are defective. The disc in D is indistinguishable from wild-type. (E and F) LamA25/Df(2L)cl-h4 hemizygotes are shown. Severe eye morphology (E) defects and nuclear migration (F) defects are observed. (G and H) LamAri3 homozygotes. The eye morphology (G) and nuclear migration (H) defects are more severe than in LamA25 hemizygotes. (I and J) LamAri3/Df(2L)cl-h4 hemizygotes. The adult eye and disc defects are similar to those of LamAri3 homozygotes. The red arrows in B, D, F, H, and J indicate the morphogenetic furrow. (K) A confocal image of a Lam4643 homozygous eye disc generated by mitotic recombination is shown, labeled with anti-Elav to mark R-cell nuclei, and phalloidin to mark apical and basal cell membranes. Size bar in J is ∼20 μm in all panels except B, D, and F, where it is ∼25 μm.