Figure 5. Lithium rescues depression-like behavior in GluD1 KO.
A. Open field test was performed on four groups, control diet WT (n = 7), lithium WT (n = 9), control diet GluD1 KO (n = 8) and lithium GluD1 KO (n = 7). In the open field test no drug X genotype effect was observed in the number of line crossings (two-way ANOVA, drug F(1, 27) = 0.8275, P = 0.3710; genotype F(1, 27) = 5.678, P = 0.0245; interaction F(1, 27) = 2.589, P = 0.1192). B. Forced swim test was performed on control diet WT (n = 12), lithium WT (n = 13), control diet GluD1 KO (n = 11) and lithium GluD1 KO (n = 11). Lithium reduced the immobility time in forced swim test in the GluD1 KO (two-way ANOVA, drug F(1, 43) = 9.717, P = 0.0033; genotype F(1, 43) = 80.49, P<0.0001; interaction F(1, 43) = 15.84, P = 0.0003). C. Resident-intruder test was performed on control diet WT (n = 5), lithium WT (n = 5), control diet GluD1 KO (n = 6) and lithium GluD1 KO (n = 5). Lithium failed to rescue the higher attack frequency in GluD1 KO in the resident-intruder test (attack frequency: two-way ANOVA, drug F(1, 17) = 0.3570, P = 0.5581; genotype F(1, 17) = 17.12, P = 0.0007; interaction F(1, 17) = 0.9182, P = 0.3514). Data are presented as mean ± SEM. *** represents P<0.001.