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. Author manuscript; available in PMC: 2012 Mar 16.
Published in final edited form as: Nature. 2005 Nov 6;439(7074):358–362. doi: 10.1038/nature04304

Figure 3. MEK inhibition causes loss of D-cyclin expression, RB hypophosphorylation and G1 arrest in BRAF mutant cancer cells.

Figure 3

a, Immunoblot showing the kinetics of change in p-ERK, D-cyclin expression and RB in SKMEL28 cells treated with 1 µM CI-1040. b, CI-1040 induced a G1 growth arrest in BRAF mutant tumour cells but not in RAS/BRAF-WT breast cancer cells (BT-474 shown). c, d, CI-1040 induces apoptosis in some but not all cancer cell lines with the BRAF(V600E) mutation as measured by FACS analysis (c) and PARP cleavage (d).