Figure 1.
Activators of the Nrf2-ARE pathway increase EAAT3 expression at the transcriptional level. A, B, Cells were exposed for 48 h to SR or t-BHQ, and the nuclear accumulation of Nrf2 (A) and EAAT3 expression (B) were assessed by Western blot. C, Nrf2 and EAAT3 band densities were normalized to the corresponding PARP-1 and actin band densities, respectively, and expressed as the percentage increase over controls (CTR). n = 3–8. *p < 0.05, **p < 0.005 versus CTR. D, Immunostaining for EAAT3 and HO-1 in cells exposed to SR or t-BHQ. Scale bar, 10 μm. E, EAAT3 promoter activity was assessed using cells exposed to SR and t-BHQ and transfected with the pEAAT3-luc and pCMV-RL plasmids. n = 3–4. *p < 0.05 versus CTR.