FIGURE 7.
NKT cell activation signals through the IL-4/STAT6 axis in adipose tissue. 6–7-Week-old mice were placed on either LFD or HFD for 4 days with two αGalCer or vehicle (Veh) injections prior to metabolic phenotyping. A, weights of body and epididymal adipose tissues. B and C, GTT, n = 7–8 mice each cohort with two repeats. D, Q-PCR analysis of M1 and M2 genes in WAT. n = 6 mice each cohort with two repeats. E and F, Western blot analysis of Arg1, (Tyr(P)) STAT6 and (Tyr(P)) STAT3 in WAT of WT and IL-4−/− mice (n = 3–5 mice each cohort with two repeats). The ratios of Arg1 to HSP90, Tyr(P) STAT3/6 to total STAT3/6 were quantitated in F. G, Western blot analyses of Tyr(P) STAT6 proteins in the liver of the WT mice under 4-day HFD with or without αGalCer injection (n = 4–5 mice each). Each lane represents an independent sample. Values represent mean ± S.E. *, p < 0.05; **, p < 0.01; ***, p < 0.005; n.s., not significant; a.u., arbitrary units.