Skip to main content
. Author manuscript; available in PMC: 2013 May 4.
Published in final edited form as: Cell Stem Cell. 2012 May 4;10(5):520–530. doi: 10.1016/j.stem.2012.04.007

Figure 5. Pharmacological targeting of Cdc42 activity restores the level and the spatial distribution of histone H4 lysine 16 acetylation.

Figure 5

(A), Representative FACS density plots of AcH4K16 expression in LT-HSCs, ST-HSCs and LMPPs. Aged LT-HSCs distribute in two distinct subpopulations expressing low and high AcH4K16 levels. (B–C), Percentage of LT-HSCs expressing low (B) and high (C) levels of AcH4K16 according to the gating shown in (A). (D–E), Median fluorescence intensity detected for AcH4K16 (D) and total AcH4 (E) in LT-HSCs, ST-HSCs and LMPPs. Shown are mean values +1 SEM, n=3. * p < 0.05. (F), Representative distribution of AcH4K16 (red) and tubulin (green) in young, aged and aged LT-HSCs treated with 5µM CASIN. Nuclei are stained with DAPI (blue). Bar = 5µm. Schemes of Tubulin and AcH4K16 distributions in young, aged and aged LT-HSCs treated with 5µM CASIN and relative fluorescence intensity plot obtained by collecting pixel intensity through the section of the relative cell are also shown. (G), Percentages of cells polarized for AcH4K16 and tubulin in young, aged and aged CASIN treated (5µM) LT-HSCs. For each sample cells were singularly analyzed and scored for AcH4K16 and tubulin polar distribution. The percentage of polarized cells is plotted over the total number of cells scored. (H), Percentages of cells polarized for AcH4K16 and tubulin in opposite poles in young, aged and aged CASIN treated (5µM) LT-HSCs. The percentage of cells showing opposite polarity is plotted over the number of cells polarized for both markers. Shown are mean mean +1 S.E., n=3, ~100–150 cells scored per sample in total. * p < 0.01. See also Figure S5 and Movies S4–S5.