Figure 2.
PABA/NO-induced S-glutathionylation of PDI in vitro. (a) MCF7 cells were seeded in a 96-well plate and treated with 0–50 μM PABA/NO. Cell viability was measured at 72 h with the MTT assay [14]. Data represent the mean for 3 independent experiments ± S.D. (b) MCF7 cells were treated with DMSO or PABA/NO at the indicated concentrations for 2 h. (c) MCF7 cells were treated with DMSO or 20 μM PABA/NO for the indicated times. Cell lysates (40 μg) were separated and under nonreducing conditions and subjected to Western blot with anti-S-glutathionylation (P-SSG) and antiactin antibodies.