Abstract
The size of transcription units for several of the abundant cytoplasmic mRNA species in mouse myeloma cells has been analyzed by the ultraviolet light mapping technique. Inactivation kinetics and target size analyses for production of the predominant RNA species indicate that the mRNAs originate in precursor molecules that are 2--14 times larger than the mature mRNA. This estimate of the size of the transcription unit may be a minimum one since it would not take into account promoter-distal sequences if these were not necessary for processing of the mRNA precursor.
Full text
PDF




Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Adesnik M., Salditt M., Thomas W., Darnell J. E. Evidence that all messenger RNA molecules (except histone messenger RNA) contain Poly (A) sequences and that the Poly(A) has a nuclear function. J Mol Biol. 1972 Oct 28;71(1):21–30. doi: 10.1016/0022-2836(72)90397-x. [DOI] [PubMed] [Google Scholar]
- Bachenheimer S., Darnell J. E. Adenovirus-2 mRNA is transcribed as part of a high-molecular-weight precursor RNA. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4445–4449. doi: 10.1073/pnas.72.11.4445. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bastos R. N., Aviv H. Globin RNA precursor molecules: biosynthesis and process in erythroid cells. Cell. 1977 Jul;11(3):641–650. doi: 10.1016/0092-8674(77)90081-2. [DOI] [PubMed] [Google Scholar]
- Berk A. J., Sharp P. A. Ultraviolet mapping of the adenovirus 2 early promoters. Cell. 1977 Sep;12(1):45–55. doi: 10.1016/0092-8674(77)90184-2. [DOI] [PubMed] [Google Scholar]
- Brack C., Tonegawa S. Variable and constant parts of the immunoglobulin light chain gene of a mouse myeloma cell are 1250 nontranslated bases apart. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5652–5656. doi: 10.1073/pnas.74.12.5652. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Breathnach R., Mandel J. L., Chambon P. Ovalbumin gene is split in chicken DNA. Nature. 1977 Nov 24;270(5635):314–319. doi: 10.1038/270314a0. [DOI] [PubMed] [Google Scholar]
- Campo M. S., Bishop J. O. Two classes of messenger RNA in cultured rat cells: repetitive sequence transcripts and unique sequence transcripts. J Mol Biol. 1974 Dec 25;90(4):649–663. doi: 10.1016/0022-2836(74)90530-0. [DOI] [PubMed] [Google Scholar]
- Carlson J., Ott G., Sauerbier W. Transcriptional organization of the Drosophila melanogaster ribosomal RNA genes. J Mol Biol. 1977 May 15;112(2):353–357. doi: 10.1016/s0022-2836(77)80151-4. [DOI] [PubMed] [Google Scholar]
- Curtis P. J., Weissmann C. Purification of globin messenger RNA from dimethylsulfoxide-induced Friend cells and detection of a putative globin messenger RNA precursor. J Mol Biol. 1976 Oct 5;106(4):1067–1075. doi: 10.1016/0022-2836(76)90353-3. [DOI] [PubMed] [Google Scholar]
- Doel M. T., Houghton M., Cook E. A., Carey N. H. The presence of ovalbumin mRNA coding sequences in multiple restriction fragments of chicken DNA. Nucleic Acids Res. 1977 Nov;4(11):3701–3713. doi: 10.1093/nar/4.11.3701. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gilmore-Hebert M., Wall R. Immunoglobulin light chain mRNA is processed from large nuclear RNA. Proc Natl Acad Sci U S A. 1978 Jan;75(1):342–345. doi: 10.1073/pnas.75.1.342. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Giorno R., Sauerbier W. A radiological analysis of the transcription units for heterogeneous nuclear RNA in cultured murine cells. Cell. 1976 Dec;9(4 Pt 2):775–783. doi: 10.1016/0092-8674(76)90140-9. [DOI] [PubMed] [Google Scholar]
- Goldberg S., Nevins J., Darnell J. E. Evidence from UV transcription mapping that late adenovirus type 2 mRNA is derived from a large precursor molecule. J Virol. 1978 Mar;25(3):806–810. doi: 10.1128/jvi.25.3.806-810.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goldberg S., Schwartz H., Darnell J. E., Jr Evidence from UV transcription mapping in HeLa cells that heterogeneous nuclear RNA is the messenger RNA precursor. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4520–4523. doi: 10.1073/pnas.74.10.4520. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goldberg S., Weber J., Darnell J. E., Jr The definition of a large viral transcription unit late in Ad2 infection of HeLa cells: mapping by effects of ultraviolet irradiation. Cell. 1977 Apr;10(4):617–621. doi: 10.1016/0092-8674(77)90094-0. [DOI] [PubMed] [Google Scholar]
- Hackett P. B., Sauerbier W. The transcriptional organization of the ribosomal RNA genes in mouse L cells. J Mol Biol. 1975 Jan 25;91(3):235–256. doi: 10.1016/0022-2836(75)90378-2. [DOI] [PubMed] [Google Scholar]
- Jeffreys A. J., Flavell R. A. The rabbit beta-globin gene contains a large large insert in the coding sequence. Cell. 1977 Dec;12(4):1097–1108. doi: 10.1016/0092-8674(77)90172-6. [DOI] [PubMed] [Google Scholar]
- Laskey R. A., Mills A. D. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975 Aug 15;56(2):335–341. doi: 10.1111/j.1432-1033.1975.tb02238.x. [DOI] [PubMed] [Google Scholar]
- Lewin B. Units of transcription and translation: sequence components of heterogeneous nuclear RNA and messenger RNA. Cell. 1975 Feb;4(2):77–93. doi: 10.1016/0092-8674(75)90113-0. [DOI] [PubMed] [Google Scholar]
- Lewin B. Units of transcription and translation: the relationship between heterogeneous nuclear RNA and messenger RNA. Cell. 1975 Jan;4(1):11–20. doi: 10.1016/0092-8674(75)90128-2. [DOI] [PubMed] [Google Scholar]
- Melli M., Spinelli G., Wyssling H., Arnold E. Presence of histone mRNA sequences in high molecular weight RNA of HeLa cells. Cell. 1977 Jul;11(3):651–661. doi: 10.1016/0092-8674(77)90082-4. [DOI] [PubMed] [Google Scholar]
- Melli M., Spinelli G., Wyssling H., Arnold E. Presence of histone mRNA sequences in high molecular weight RNA of HeLa cells. Cell. 1977 Jul;11(3):651–661. doi: 10.1016/0092-8674(77)90082-4. [DOI] [PubMed] [Google Scholar]
- NYGAARD A. P., HALL B. D. A method for the detection of RNA-DNA complexes. Biochem Biophys Res Commun. 1963 Jul 18;12:98–104. doi: 10.1016/0006-291x(63)90242-0. [DOI] [PubMed] [Google Scholar]
- Rabbitts T. H., Forster A. Evidence for noncontiguous variable and constant region genes in both germ line and myeloma DNA. Cell. 1978 Feb;13(2):319–327. doi: 10.1016/0092-8674(78)90200-3. [DOI] [PubMed] [Google Scholar]
- Ross J. A precursor of globin messenger RNA. J Mol Biol. 1976 Sep 15;106(2):403–420. doi: 10.1016/0022-2836(76)90093-0. [DOI] [PubMed] [Google Scholar]
- Spradling A., Pardue M. L., Penman S. Messenger RNA in heat-shocked Drosophila cells. J Mol Biol. 1977 Feb 5;109(4):559–587. doi: 10.1016/s0022-2836(77)80091-0. [DOI] [PubMed] [Google Scholar]
- Tilghman S. M., Curtis P. J., Tiemeier D. C., Leder P., Weissmann C. The intervening sequence of a mouse beta-globin gene is transcribed within the 15S beta-globin mRNA precursor. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1309–1313. doi: 10.1073/pnas.75.3.1309. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vogelstein B., Gillespie D. RNA-DNA hybridization in solution without DNA reannealing. Biochem Biophys Res Commun. 1977 Apr 25;75(4):1127–1132. doi: 10.1016/0006-291x(77)91500-5. [DOI] [PubMed] [Google Scholar]