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. 1978 Sep;75(9):4519–4523. doi: 10.1073/pnas.75.9.4519

Formation of high density lipoprotein2-like particles during lipolysis of very low density lipoproteins in vitro.

J R Patsch, A M Gotto Jr, T Olivercrona, S Eisenberg
PMCID: PMC336147  PMID: 212757

Abstract

The effects of lipolysis of human plasma very low density lipoprotein (VLDL) on the structure and composition of high density lipoproteins (HDL) have been investigated. Lipolysis was performed in a controlled system in vitro containing VLDL (d less than 1.006 g/ml) and HDL3 (d = 1.125-1.210 g/ml) from human plasma and lipoprotein lipase (EC 3.1.1.34) purified from bovine milk. Lipolysis of VLDL caused profound changes in HDL3. Protein, phospholipid, and cholesterol liberated from VLDL during its lipolysis were transferred to the HDL3 particles. As a consequence of this in vitro transfer, the chemical composition and biophysical properties of HDL3 were substantially altered. The newly formed particles exhibited a flotation rate (F01.20) of 6.7 and a hydrated density of 1.110 g/ml. The chemical composition closely resembled that of native HDL2, and their size was slightly larger than that of the precursor HDL3. When HDL3 and postlipolysis HDL2 were subjected to ultracentrifugation under flotation velocity and equilibrium conditions, both proved to be stable particles. These results, when extrapolated to in vivo conditions, suggest an important metabolic relationship between the levels of circulating VLDL and HDL2 in plasma. This relationship now permits a reasonable explanation for numerous in vivo observations in which the levels of VLDL and HDL2 change reciprocally.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson D. W., Nichols A. V., Forte T. M., Lindgren F. T. Particle distribution of human serum high density lipoproteins. Biochim Biophys Acta. 1977 Jul 22;493(1):55–68. doi: 10.1016/0005-2795(77)90259-8. [DOI] [PubMed] [Google Scholar]
  2. Applebaum D. M., Goldberg A. P., Pykälistö O. J., Brunzell J. D., Hazzard W. R. Effect of estrogen on post-heparin lipolytic activity. Selective decline in hepatic triglyceride lipase. J Clin Invest. 1977 Apr;59(4):601–608. doi: 10.1172/JCI108677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BARTLETT G. R. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed] [Google Scholar]
  4. Bengtsson G., Olivecrona T. Interaction of lipoprotein lipase with heparin-Sepharose. Evaluation of conditions for affinity binding. Biochem J. 1977 Oct 1;167(1):109–119. doi: 10.1042/bj1670109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bilheimer D. W., Eisenberg S., Levy R. I. The metabolism of very low density lipoprotein proteins. I. Preliminary in vitro and in vivo observations. Biochim Biophys Acta. 1972 Feb 21;260(2):212–221. doi: 10.1016/0005-2760(72)90034-3. [DOI] [PubMed] [Google Scholar]
  6. Carew T. E., Koschinsky T., Hayes S. B., Steinberg D. A mechanism by which high-density lipoproteins may slow the atherogenic process. Lancet. 1976 Jun 19;1(7973):1315–1317. doi: 10.1016/s0140-6736(76)92650-7. [DOI] [PubMed] [Google Scholar]
  7. Chajek T., Eisenberg S. Very low density lipoprotein. Metabolism of phospholipids, cholesterol, and apolipoprotein C in the isolated perfused rat heart. J Clin Invest. 1978 Jun;61(6):1654–1665. doi: 10.1172/JCI109086. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Eisenberg S., Bilheimer D. W., Levy R. I., Lindgren F. T. On the metabolic conversion of human plasma very low density lipoprotein to low density lipoprotein. Biochim Biophys Acta. 1973 Dec 20;326(3):361–377. doi: 10.1016/0005-2760(73)90138-0. [DOI] [PubMed] [Google Scholar]
  9. Eisenberg S., Schurr D. Phospholipid removal during degradation of rat plasma very low density lipoprotein in vitro. J Lipid Res. 1976 Nov;17(6):578–587. [PubMed] [Google Scholar]
  10. Fredrickson D. S., Levy R. I., Lindgren F. T. A comparison of heritable abnormal lipoprotein patterns as defined by two different techniques. J Clin Invest. 1969 Nov;47(11):2446–2457. doi: 10.1172/JCI105927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Glueck C. J., Fallat R. W., Scheel D. Effects of estrogenic compounds on triglyceride kinetics. Metabolism. 1975 Apr;24(4):537–545. doi: 10.1016/0026-0495(75)90078-5. [DOI] [PubMed] [Google Scholar]
  12. Gordon T., Castelli W. P., Hjortland M. C., Kannel W. B., Dawber T. R. High density lipoprotein as a protective factor against coronary heart disease. The Framingham Study. Am J Med. 1977 May;62(5):707–714. doi: 10.1016/0002-9343(77)90874-9. [DOI] [PubMed] [Google Scholar]
  13. Green P. H., Tall A. R., Glickman R. M. Rat intestine secretes discoid high density lipoprotein. J Clin Invest. 1978 Feb;61(2):528–534. doi: 10.1172/JCI108963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hamilton R. L., Williams M. C., Fielding C. J., Havel R. J. Discoidal bilayer structure of nascent high density lipoproteins from perfused rat liver. J Clin Invest. 1976 Sep;58(3):667–680. doi: 10.1172/JCI108513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Havel R. J., Kane J. P., Kashyap M. L. Interchange of apolipoproteins between chylomicrons and high density lipoproteins during alimentary lipemia in man. J Clin Invest. 1973 Jan;52(1):32–38. doi: 10.1172/JCI107171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kostner G., Alaupovic P. Studies of the composition and structure of plasma lipoproteins. Separation and quantification of the lipoprotein families occurring in the high density lipoproteins of human plasma. Biochemistry. 1972 Aug 29;11(18):3419–3428. doi: 10.1021/bi00768a015. [DOI] [PubMed] [Google Scholar]
  17. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  18. LaRosa J. C., Levy R. I., Brown W. V., Fredrickson D. S. Changes in high-density lipoprotein protein composition after heparin-induced lipolysis. Am J Physiol. 1971 Mar;220(3):785–791. doi: 10.1152/ajplegacy.1971.220.3.785. [DOI] [PubMed] [Google Scholar]
  19. Miller K. L. Post delivery care of the edentulous geriatric patient. Bull Phila Cty Dent Soc. 1975 Summer;40(8):16–19. [PubMed] [Google Scholar]
  20. Noble R. P. Electrophoretic separation of plasma lipoproteins in agarose gel. J Lipid Res. 1968 Nov;9(6):693–700. [PubMed] [Google Scholar]
  21. Patsch J. R., Aune K. C., Gotto A. M., Jr, Morrisett J. D. Isolation, chemical characterization, and biophysical properties of three different abnormal lipoproteins: LP-X1, LP-X2, and LP-X3. J Biol Chem. 1977 Mar 25;252(6):2113–2120. [PubMed] [Google Scholar]
  22. Patsch J. R., Sailer S., Kostner G., Sandhofer F., Holasek A., Braunsteiner H. Separation of the main lipoprotein density classes from human plasma by rate-zonal ultracentrifugation. J Lipid Res. 1974 Jul;15(4):356–366. [PubMed] [Google Scholar]
  23. Patsch J. R., Yeshurun D., Jackson R. L., Gotto A. M., Jr Effects of clofibrate, nicotinic acid and diet on the properties of the plasma lipoproteins in a subject with type III hyperlipoproteinemia. Am J Med. 1977 Dec;63(6):1001–1009. doi: 10.1016/0002-9343(77)90555-1. [DOI] [PubMed] [Google Scholar]
  24. Patsch W., Patsch J. R., Kostner G. M., Sailer S., Braunsteiner H. Isolation of subfractions of human very low density lipoproteins by zonal ultracentrifugation. J Biol Chem. 1978 Jul 25;253(14):4911–4915. [PubMed] [Google Scholar]
  25. Patsch W., Sailer S., Braunsteiner H. An enzymatic method for the determination of the initial rate of cholesterol esterification in human plasma. J Lipid Res. 1976 Mar;17(2):182–185. [PubMed] [Google Scholar]
  26. Rhoads G. G., Gulbrandsen C. L., Kagan A. Serum lipoproteins and coronary heart disease in a population study of Hawaii Japanese men. N Engl J Med. 1976 Feb 5;294(6):293–298. doi: 10.1056/NEJM197602052940601. [DOI] [PubMed] [Google Scholar]
  27. Scanu A., Granda J. L. Effects of ultracentrifugation on the human serum high-density (1.063 less than p less than 1.21 g/ml) lipoprotein. Biochemistry. 1966 Feb;5(2):446–455. doi: 10.1021/bi00866a008. [DOI] [PubMed] [Google Scholar]
  28. Schaefer E. J., Jenkins L. L., Brewer H. B., Jr Human chylomicron apolipoprotein metabolism. Biochem Biophys Res Commun. 1978 Jan 30;80(2):405–412. doi: 10.1016/0006-291x(78)90691-5. [DOI] [PubMed] [Google Scholar]
  29. Wolfe B. M., Kane J. P., Havel R. J., Brewster H. P. Mechanism of the hypolipemic effect of clofibrate in postabsorptive man. J Clin Invest. 1973 Sep;52(9):2146–2159. doi: 10.1172/JCI107399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wood P. D., Haskell W., Klein H., Lewis S., Stern M. P., Farquhar J. W. The distribution of plasma lipoproteins in middle-aged male runners. Metabolism. 1976 Nov;25(11):1249–1257. doi: 10.1016/s0026-0495(76)80008-x. [DOI] [PubMed] [Google Scholar]

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