Table 2.
In Vitro Development of Cloned Embryos Reconstructed with Transgenic Donor cells by HMC
|
No. and (%) of oocytes |
|
|
|
---|---|---|---|---|
Donor cells | Total oocytes | Used for HMC | No. of reconstructed embryos | No. and (%) of cloned embryos developed to blastocysts |
CAG3-2-2, Passage 9 | 654 | 491 (75.1%) | 203 | 64 (31.5%) |
678 | 489 (72.1%) | 215 | 90 (41.9%) | |
651 | 486 (74.7%) | 214 | 71 (33.2%) | |
CAG1-3-6, Passage 8 | 791 | 658 (83.2%) | 278 | 119 (42.8%) |
825 | 647 (78.4%) | 286 | 96 (33.6%) | |
822 | 639 (77.7%) | 289 | 118 (40.8%) | |
Total | 4421 | 3410 (77.1±3.8%) | 1485 | 558 (37.6±5.1%) |
HMC, handmade cloning.