TABLE 2.
Primer sequences for real-time PCR
| Name | Forward | Reverse | Product (bp) |
|---|---|---|---|
| Gapdh | TaqMan® Rodent GAPDH Control Reagents (#4308313) from Applied Biosystems, Foster City, CA, sequences are proprietary, spanning exons 2 and 3 | 177 | |
| Nos2 (iNOS) | CAGCTGGGCTGTACAAACCTT | CATTGGAAGTGAAGCGTTTCG | 95 |
| Il18 | GACTCTTGCGTCAACTTCAAGG | CAGGCTGTCTTTTGTCAACGA | 169 |
| Tnf (TNFα) | CTACTCCCAGGTTCTCTTCAA | GCAGAGAGGAGGTTGACTTTC | 111 |
| Arg1 | TGGCTTGCGAGACGTAGAC | GCTCAGGTGAATCGGCCTTTT | 160 |
| Il10 | CTGGACAACATACTGCTAACCG | GGGCATCACTTCTACCAGGTAA | 108 |
| Ilrn (IL-1ra) | AAATCTGCTGGGGACCCTAC | TGAGCTGGTTGTTTCTCAGG | 164 |