Figure 2. Immunosensor data using the moderate sensitivity protocol.
(A) Amperometric responses of gold nanoparticle immunosensor with attached capture antibody incubated with NANOG protein standards (pg/ml) in 10 µl buffer for 1 h after treating with 20 µl of 2% bovine serum albumin for 45 min, followed by the addition of 10 µl biotinylated enzyme-labeled secondary antibody in 0.1% bovine serum albumin and 0.05% Tween-20 for 45 min, and later incubating with streptavidin–horseradish peroxidase for 30 min. The current profiles were recorded upon injection of H2O2 (0.1 mM), into a buffer solution containing the sensor with captured NANOG and detection antibodies, and 1 mM hydroquinone, with electrode potential at −0.3 V and a rotation speed of 3000 rpm. (B) Immunoarray calibration plot showing the influence of NANOG concentration on the steady-state sensor current.