Fig 6.
MeCP2 regulates PMA-induced expression of EGR2. (A) Changes in gene expression induced by treatment of SH-SY5Y cells with 16 nM PMA for the indicated times. (B) Schematic of the EGR2 gene showing the qPCR amplicons used for panels C to E. (C to E) ChIP with total MeCP2 (C), pS80 MeCP2 (D), and pS229 MeCP2 (E) antibodies analyzed by quantitative PCR at the indicated sites, expressed as enrichment over nonspecific IgY. (F) PMA-induced expression changes of EGR2 in parental SH-SY5Y or SH-SY5Y cell lines stably expressing the indicated MeCP2e1-FLAG construct. Expression was measured by quantitative PCR and normalized to GAPDH expression. Data are means from three independent biological replicates. Error bars represent SEM. *, P < 0.05 compared to the corresponding result in the SH-SY5Y parental cell line.