Fig. 4.
Mice derived from embryo culture exhibit normal spatial learning. Adult mice derived from embryos that developed either in vivo or in vitro were examined by using the hidden platform version of the Morris water maze (17, 18). ○, in vivo-derived mice; •, culture-(all mice obtained after culture in KSOM and WM)-derived mice. Error bars are omitted for clarity. Overall, when the performance of cultured embryos was compared with those that developed in vivo, there were no significant differences in learning (P > 0.05). The numbers of mice examined for in vivo, KSOM, or WM animals were 34, 47, and 46, respectively. Transgenic and nontransgenic animals (wild-type, 39; TETO, 48; and LN30, 40) were distributed among these groups in a balanced fashion, and the observed differences cannot be attributed to genotype (data not shown).