Figure 3. Behavioral detection of photostimulation.
(a) Comparison of the performance ((hits + correct rejections)/total trials) in mice expressing ChR2-GFP (n = 9) and control mice (n = 6) after training with 5 photostimuli (P < 0.001, t-test). (b) Performance as a function of light intensity (in % of Imax = 11.6 mW/mm2) for 5 light pulses (1ms, 20Hz, blue lines), 2 light pulses (1ms, 20Hz, green lines) and a single light pulse (1ms, red lines). Dotted lines: mean across 5 session (200–1000 trials per session). Error bars: binomial standard error. The number of ChR2-GFP-positive neurons located under the window area is indicated for each mouse. (c) Location of ChR2 expressing neurons in serial reconstruction of the sectioned brain (coronal sections). The blue cone illustrates the light source over the window. Arrows indicate rostral (r) and dorsal (d) orientation. (d) Performance as a function of the number of activated neurons. Thick lines, mean performance across all 5 animals for 1 (red), 2 (green) and 5 (blue) light pulses. Dotted lines indicate mean values of individual animals from Fig. 3b.