FIGURE 6:
The G2S, but not the G2V or W4A, mutant forms functional gap junctions. (A) NRK and HeLa cells were untreated (WT) or engineered to express W4A-GFP or G2S-GFP before microinjection with 5% Lucifer yellow and assessment of dye transfer. (B) Gap junctional intercellular communication–deficient N2A cells were engineered to express Cx43-GFP, W4A-GFP, G2V-GFP, or G2S-GFP. Patch-clamp analysis was used to assess gap junction coupling conductance. Whereas the W4A and G2V mutants did not form functional gap junction channels, the G2S mutant exhibited functional conductance that even exceeded that of Cx43. The numbers above each column depict the number of cell pairs recorded.