Fig 7.
Eap1p associates with HO mRNA, dependent on Puf5p. (A) FLAG-tagged wild-type Eap1p (WT) or eIF4E binding defect mutant Eap1p (mt) were expressed and immunopurified from wild-type or Δpuf5 cells. For a negative control, mock immunoprecipitation was performed on cells that did not express tagged Eap1p. The blot is an anti-FLAG Western blot of FLAG peptide eluates. (B) Graph of fold enrichment of HO mRNA purified from eluted samples in panel A, detected using qRT-PCR. Fold enrichment was calculated relative to mock immunoprecipitation, normalized to input levels. As a negative control, 18S rRNA was also measured. Note that conditions used in this assay did not preserve polyribosomes.