Figure 2.
Purified 52-kDa nitrated protein from rat kidney 6 h after LPS injection. Lane 1, molecular mass standards; lane 2, 3 μg of 52-kDa protein after Affi-Gel Blue agarose column. The molecular masses (kDa) of protein standards are indicated on the left. Coomassie-stained SDS-polyacrylamide gel (A) and Western immunoblots with Upstate Biotechnology monoclonal anti-nitrotyrosine antibody (B) or with polyclonal anti-SCOT antibody (C) are shown.