Fig. 3.
Formation of DCF-GSH conjugates in vitro and in vivo. A and B, relative rates of in vitro formation of DCF-GSH conjugate were determined for microsomal preparations from mouse SI (A) and liver (B). Reaction mixtures contained 50 mM phosphate buffer, pH 7.4, 100 μM DCF, 10 mM GSH, and 1 mg/ml microsomal protein in a final volume of 0.2 ml. Reactions were carried out at 37°C for 60 min in the presence or absence of 1 mM NADPH. Each microsomal preparation was obtained from pooled liver or SI tissues of two to three adult female mice. The values reported, which are relative rates in arbitrary units (percentages of the rates determined for respective WT microsomes), represent means ± S.D. (n = 3). C and D, relative tissue levels of DCF-GSH conjugates in SI (C) and liver (D). Adult female IE-Cpr-null and age-matched WT mice were given a single dose of DCF, via oral gavage, at 50 mg/kg. The values reported, which are percentages of the levels determined for respective WT mice, represent means ± S.D. (n = 4). **, p < 0.01; compared with WT mice (Student's t test).